CD27 antibody (APC)

Catalog No. ABIN6655549

Supplier Product No.: 200-326-n65

The Mouse Monoclonal anti-CD27 antibody has been validated for FACS. It is suitable to detect CD27 in samples from Human.

Quick Overview for CD27 antibody (APC) (ABIN6655549)

Target: CD27 (CD27 Molecule (CD27))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD27 antibody is conjugated to APC

Application: Flow Cytometry (FACS)

Clone: O323

Product Details anti-CD27 Antibody (APC)

Supplier Product No.: 200-326-n65

Supplier: Rockland

Purpose: CD27 Allophycocyanin Antibody

Cross-Reactivity (Details): Reactivity is observed against human CD27, Baboon, Cynomolgus, and Rhesus.

Purification: Allophycocyanin conjugated CD27 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD27.

Sterility: Sterile filtered

Immunogen: Anti-CD27 Antibody (Monoclonal) was produced by repeated immunizations with CD27 antigen.

Isotype: IgG1 kappa

Labeling Ratio: 1-2

Application Details

Application Notes: Optional[Neutralization_Dilution]: 5μL/test/1x10e5 to 1x10e8 cells

Comment:

Anti-CD27 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 0.1 % Gelatin

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiry Date: 6 months

Target Details for CD27

Target: CD27 (CD27 Molecule (CD27))

Alternative Name: CD27

Background:

Synonyms: CD27 antigen, CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, CD27, TNFRSF7

Background: CD27 antibody reacts with human CD27 (TNFRSF7), a cell surface homodimer of 55 kDa subunits, which provides co-stimulatory signaling in support of the T cell (TCR) and B cell (BCR) receptors. By comparison with CD28, whose TCR co-stimulatory signal can trigger cell proliferation, CD27 signaling appears to promote cell survival and differentiation to effector / memory stages. Also in contrast with CD28, the CD27 receptor may be shed following interaction with its ligand CD70, which is typically expressed on activated dendritic cells, T cells and B cells. With respect to B cells, CD27 is considered to be a phenotypic marker for memory B cells. CD27 has been included within a group of phenotypic markers for identifying human B regulatory cells (Bregs), a cell type proposed to regulate CD4+ T cell proliferation and Foxp3 / CTLA-4 expression in Treg cells. The CD27 antibody may be used for analysis of CD27 expression on peripheral T cells, and is frequently used in combination with antibodies for IgD and IgM to distinguish naive vs. memory B cell populations. For identification of Breg cells, this antibody has been used in combination with antibodies for CD25, CD27, CD1d, IL-10 and TGF-beta.

Gene Name: CD27

Gene ID: 939

NCBI Accession: NP_001233

UniProt: P26842

Pathways: Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints